spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by FENWICK, M. L.
Right arrow Articles by WALL, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by FENWICK, M. L.
Right arrow Articles by WALL, M. J.

Journal of Cell Science, Vol 13, 403-413, Copyright © 1973 by Company of Biologists

Revised on January 19, 1973

Factors Determining the Site of Synthesis of Polio Virus Proteins: The Early Attachment of Virus Particles to Endoplasmic Membranes

M. L. FENWICK 1 and MARGARET J. WALL 1

1 Sir William Dunn School of Pathology, University of Oxford, England

Cytoplasmic extracts of HeLa cells made 1 to 2 h after infection with radioactive poliovirus contained 150-S virus particles and 130-S, perhaps partially disrupted, particles. The latter were resistant to RNase but sensitive to dodecylsulphate. Both particles were associated with fast sedimenting material from which they could be released by deoxycholate, but not by EDTA. In isopycnic gradients of sucrose in D2O the labelled particles formed a sharp band coincident with membrane-bound ribosomes at a density of 1.23 g cm-3. It is suggested that attachment of virus particles to endoplasmic reticulum may be an early stage in the infectious cycle, determining the site of subsequent steps.

The inhibition of cellular protein synthesis that develops during infection affects membrane-bound as well as free polysomes and therefore does not determine the membrane-association of viral protein synthesis.

Revised on January 19, 1973




This article has been cited by other articles:


Home page
 Mol. Pathol.Home page
M S Smyth and J H Martin
Picornavirus uncoating
Mol. Pathol., August 1, 2002; 55(4): 214 - 219.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. Basavappa, A. Gomez-Yafal, and J. M. Hogle
The Poliovirus Empty Capsid Specifically Recognizes the Poliovirus Receptor and Undergoes Some, but Not All, of the Transitions Associated with Cell Entry
J. Virol., September 1, 1998; 72(9): 7551 - 7556.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1973