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Journal of Cell Science, Vol 13, 591-601, Copyright © 1973 by Company of Biologists

Submitted on December 5, 1972

Peroxidases in Tobacco Abscission Zone Tissue

I. Fine-Structural Localization in Cell Walls During Ethylene-Induced Abscission

E. W. HENRY 1 and T. E. JENSEN 1

1 Department of Biological Sciences, Herbert H. Lehman College of the City University of New York, Bronx, New York 10468, U.S.A.

The fine-structural localization of peroxidases during ethylene-induced abscission of flower pedicels of Nicotiana tabacum L. cv. ‘Little Turkish’ has been investigated. Peroxidase activity has been localized in both the cell walls and intercellular spaces of ethylene-treated flower pedicels which were fixed in glutaraldehyde, incubated in diaminobenzidine (DAB) medium with postfixation in 2% osmium tetroxide. Peroxidase staining is present in the cell walls and intercellular spaces of control tissue but is not as intense as in ethylene-treated tissue. Increased peroxidase staining is evident in the intercellular spaces and cell walls after 2 h of exposure to ethylene and increases in intensity between 2 and 5 h. At 5 h, ethylene-induced abscission occurs. Fine-structural investigations revealed prominent staining in the middle-lamellar and peripheral areas of the cell walls in ethylene-treated tissue. The peroxidase staining appears to be due to peroxidase as prior incubation with potassium cyanide gives a marked reduction in the staining reaction. Incubation of the ethylene-exposed tissue in aminotriazole, a specific inhibitor of catalase, does not reduce peroxidase staining, except in the microbodies, which reportedly contain catalase.

Submitted on December 5, 1972






© The Company of Biologists Ltd 1973