spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by NYORMOI, O.
Right arrow Articles by SINCLAIR, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by NYORMOI, O.
Right arrow Articles by SINCLAIR, J. H.

Journal of Cell Science, Vol 13, 863-878, Copyright © 1973 by Company of Biologists

Proliferating Hybrid Cells Formed Between Rat Spermatids and An Established Line of Mouse Fibroblasts

OKOT NYORMOI 1 and J. H. SINCLAIR 2

1 Department of Zoology, Indiana University Bloomington, Indiana 47401, U.S.A.; Uganda Cancer Institute, P.O. Box 3935, Kampala, Uganda
2 Department of Zoology, Indiana University Bloomington, Indiana 47401, U.S.A.; Laboratory of Cell Biology, National Institutes of Health, Bethesda, Maryland 20014, U.S.A.

Proliferating hybrids were formed between rat spermatids and LM(TK-)cl 1 D, an established line of mouse fibroblasts. Spermatids were obtained from dissociated rat seminiferous tubules by differential attachment of testicular somatic cells to culture plates, followed by gravity velocity sedimentation into linear Ficoll gradients. Cells were fused with the aid of Sendai virus and hybrid selection was facilitated by the inability of cl 1 D cells to grow in HAT medium and the inability of spermatids to attach or proliferate in vitro. Three types of cells grew in fusion plates: (1) unfused contaminant rat testicular somatic cells; (2) rat testicular cell x cl 1 D hybrids and (3) rat spermatid x cl 1 D hybrids. Unfused rat testicular somatic cells were morphologically and karyologically different from either type of hybrid. The 2 hybrid classes were distinguished by the total number of chromosomes and the number of parental chromosome markers. Rat chromosomes are preferentially eliminated from haploid as well as diploid hybrids and at least 3 rat enzymes (lactate dehydrogenase, glucose phosphate isomerase and an unknown oxidase) are synthesized in hybrid clones of both types. The implications of partial haploid hybrids for genetic analysis are briefly discussed.






© The Company of Biologists Ltd 1973