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Journal of Cell Science, Vol 14, 139-161, Copyright © 1974 by Company of Biologists

Submitted on June 1, 1973

Cathepsin D in Cartilage: The Immunohistochemical Demonstration of Extracellular Enzyme in Normal and Pathological Conditions

A. R. POOLE 1, R. M. HEMBRY 1, and J. T. DINGLE 1

1 Tissue Physiology Department, Strangeways Research Laboratory, Wort's Causeway, Cambridge, CB1 4RN, England

Extracellular cathepsin D has been demonstrated immunohistochemically in cultured chicken and rabbit cartilage. Enzyme released from viable cells was captured by specific anti-cathepsin D antibody to form extracellular immunoprecipitates which were demonstrated with fluorescein-labelled antibodies. In unfixed frozen sections these immunoprecipitates could be dissolved in antigen excess, and closely resembled those observed in immunoprecipitin lines. Extracellular cathepsin D was detected in the perichondria of normal chick cartilaginous limb-bones and, to a lesser degree, beneath them in the epiphyseal cartilage. In cultured rabbit ear cartilage, cathepsin D was detected around chondroblasts and some peripherally situated chondrocytes. In chicken and rabbit cartilage exposed to supra-physiological levels of retinol, much extracellular enzyme was detected which had been released from chondrocytes of hypertrophic and epiphyseal chick cartilage and rabbit ear cartilage.

Submitted on June 1, 1973




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© The Company of Biologists Ltd 1974