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Journal of Cell Science, Vol 14, 203-214, Copyright © 1974 by Company of Biologists

Submitted on January 26, 1973
Revised on May 11, 1973

Surface Modification and Electrophoresis of Normal and Transformed BHK21 Cells

A. L. LATNER 1 and G. A. TURNER 1

1 Cancer Research Unit, University Department of Clinical Biochemistry, Royal Victoria Infirmary Newcastle upon Tyne, England

The mean electrophoretic mobilities at pH 7.5 of virus-transformed (Py6) and normal BHK21 cells were very similar, whether they were harvested mechanically or by the use of trypsin. After formaldehyde treatment, there was significantly increased mobility in both cell types; the transformed cells showed significantly the greater change. After neuraminidase treatment, the mean electrophoretic mobility was decreased to the same extent in both types of cell. Treatment with neuraminidase and formaldehyde had no effect on the mean electrophoretic mobility of the normal cells but slowed the transformed variety. The mobility in histone solution had no relationship to histone concentration but was statistically correlated with the amount of histone per cell, calculated from total histone present divided by the total number of cells; a linear relationship being obtained with the normal cells but an initial plateau was demonstrated with the transformed cells. The normal cell line showed a similar plateau after neuraminidase treatment. The possible significance of these results is discussed.

Submitted on January 26, 1973
Revised on May 11, 1973


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© The Company of Biologists Ltd 1974