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Journal of Cell Science, Vol 16, 133-142, Copyright © 1974 by Company of Biologists

Submitted on February 20, 1974

Studies on the Template Activity of ‘Isolated" Xenopus Erythrocyte Nuclei

I. The Effects of Ions

V. A. HILDER 1 and N. MACLEAN 1

1 Department of Biology, University of Southampton, Southampton SO9 3 TU, England

The isolation and characterization of nuclei from Xenopus erythrocytes is described. Such nuclei are freely permeable to ions and large molecules. They have an extremely low basal rate of RNA synthesis which can be increased by the addition of Escherichia coli RNA polymerase and by alteration in the ionic strength of the incubation medium within physiological limits. The results are discussed in terms of the mechanisms which control gene activity in these cells.

Submitted on February 20, 1974






© The Company of Biologists Ltd 1974