spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by BENBASSAT, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by BENBASSAT, J.

Journal of Cell Science, Vol 16, 143-156, Copyright © 1974 by Company of Biologists

Submitted on February 15, 1974

The Transition from Tadpole to Frog Haemoglobin during Natural Amphibian Metamorphosis

II. Immunofluorescence Studies

J. BENBASSAT 1

1 Department of Medicine A, Hadassah University Hospital, Jerusalem, Israel

Rabbits were immunized with frog or tadpole haemoglobin purified either by chromatography or by polyacrylamide gel electrophoresis. The obtained rabbit antisera were shown to be specific for frog or tadpole haemolysates by double diffusion, immunoprecipitation and immunofluorescence. Indirect immunofluorescent staining of peripheral blood smears of Rana catesbeiana tadpoles at the metamorphic climax revealed that 16% of the red cells were stained with antibodies against frog haemolysates, while almost all of them (98%) were stained with antibodies against tadpole haemolysates. These results are compatible with the possibility that some of the circulating red cells in metamorphosing tadpoles contain both tadpole and frog haemoglobins.

Submitted on February 15, 1974






© The Company of Biologists Ltd 1974