spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by GORDON, J. L.
Right arrow Articles by DINGLE, J. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by GORDON, J. L.
Right arrow Articles by DINGLE, J. T.

Journal of Cell Science, Vol 16, 157-166, Copyright © 1974 by Company of Biologists

Submitted on February 6, 1974

Binding of Radiolabelled Collagen to Blood Platelets

J. L. GORDON 1 and J. T. DINGLE 1

1 Department of Pathology, Cambridge University, Tennis Court Road, Cambridge, CB2 1QP, and Tissue Physiology Department, Strangeways Research Laboratory, Wort's Causeway, Cambridge, CB1 4RN, England

Purified, soluble collagen was prepared from rat skin, and the tyrosine residues were labelled with 125I by the chloramine T method. The collagen was added to platelet-rich plasma from rats or rabbits, and then layered on 23 % w/v Ficoll. After centrifugation at 3900 g for 20 min, the platelets were recovered from beneath the Ficoll, and the radioactivity associated with them was measured. This represented the platelet-bound collagen, since very little collagen penetrated the Ficoll if platelets were absent.

The amount of platelet-bound collagen paralleled the extent of collagen microfibril formation, which in turn increased with the time the collagen was preincubated at 36 °C before being added to the plasma.

The amount of binding in each sample was reproducible within groups of rats or rabbits, but the amount of collagen bound per platelet was about 3 times greater in samples from rabbits than from rats.

Submitted on February 6, 1974




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. M. Jung and M. Moroi
Platelets Interact with Soluble and Insoluble Collagens through Characteristically Different Reactions
J. Biol. Chem., June 12, 1998; 273(24): 14827 - 14837.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1974