|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 16, 349-358, Copyright © 1974 by Company of Biologists
Submitted on March 4, 1974
1 Max-Planck-Institut für Virusforschung, Molekularbiologische Abteilung, Tübingen, Germany, and Department of Developmental and Cell Biology, University of California, Irvine, California 92664, U.S.A.; Department of Developmental and Cell Biology, University of California, Irvine, California 92664, U.S.A.
2 Max-Planck-Institut für Virusforschung, Molekularbiologische Abteilung, Tübingen, Germany, and Department of Developmental and Cell Biology, University of California, Irvine, California 92664, U.S.A.; Department of Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, U.S.A.
The cell cycle parameters of interstitial cells in Hydra attenuata have been determined. Interstitial cells were classified according to cluster size in which they occur (1, 2, 4, 8 or 16 cells) and morphology using maceration preparations and histological sections. The lengths of G1, S, G2 and M were determined by standard methods of cell cycle analysis using pulse-chase and continuous labelling with [3H]- and [14C]thymidine. Nuclear DNA contents were measured microfluorimetrically. All classes of interstitial cells proliferate but the cell cycle of large interstitial cells occurring singly or in pairs is longer than that of interstitial cells occurring in clusters of 4, 8 and 16 cells. The S-phase is 11-12 h long and G1 is less than 1 h for all classes of interstitial cells. G2 is 3-4 h long for interstitial cells in clusters of 4, 8 and 16 cells giving these cells a total cell cycle duration of 16-17 h. In contrast, large interstitial cells occurring as singles and in clusters of 2 have G2 durations ranging from 4 to 22 h. Two subpopulations can be discerned among these cells, one having a G1 of about 6 h and a total cell cycle of about 19 h, the other having an average G2 of 14 h and a total cell cycle of about 27 h. The differences in cell cycle duration appear to be associated with interstitial cell function. Cells having a short cell cycle are probably committed to nematocyte differentiation, while large interstitial cells having long and variable cell cycles appear to be undetermined stem cells responsible for proliferating further interstitial cells. The variable length of G2 in these cells suggest it as a possible control point.
Submitted on March 4, 1974
This article has been cited by other articles:
|
|
 |
|
  J. S. Hwang, H. Ohyanagi, S. Hayakawa, N. Osato, C. Nishimiya-Fujisawa, K. Ikeo, C. N. David, T. Fujisawa, and T. Gojobori The evolutionary emergence of cell type-specific genes inferred from the gene expression analysis of Hydra PNAS, September 11, 2007; 104(37): 14735 - 14740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miljkovic-Licina, S. Chera, L. Ghila, and B. Galliot Head regeneration in wild-type hydra requires de novo neurogenesis Development, March 15, 2007; 134(6): 1191 - 1201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Broun, L. Gee, B. Reinhardt, and H. R. Bode Formation of the head organizer in hydra involves the canonical Wnt pathway Development, June 15, 2005; 132(12): 2907 - 2916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takahashi, M. Hatta, S. Yum, L. Gee, M. Ohtani, T. Fujisawa, and H. R. Bode Hym-301, a novel peptide, regulates the number of tentacles formed in hydra Development, May 1, 2005; 132(9): 2225 - 2234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Lindgens, T. W. Holstein, and U. Technau Hyzic, the Hydra homolog of the zic/odd-paired gene, is involved in the early specification of the sensory nematocytes Development, January 1, 2004; 131(1): 191 - 201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N Harafuji, T Takahashi, M Hatta, H Tezuka, F Morishita, O Matsushima, and T Fujisawa Enhancement of foot formation in Hydra by a novel epitheliopeptide, Hym-323 Development, January 2, 2001; 128(3): 437 - 446. [Abstract] [PDF]
|
|
|
|
|
|
T Takahashi, O Koizumi, Y Ariura, A Romanovitch, T. Bosch, Y Kobayakawa, S Mohri, H. Bode, S Yum, M Hatta, et al. A novel neuropeptide, Hym-355, positively regulates neuron differentiation in Hydra Development, January 3, 2000; 127(5): 997 - 1005. [Abstract] [PDF]
|
|
|
|
|
|
A Grens, H Shimizu, S. Hoffmeister, H. Bode, and T Fujisawa The novel signal peptides, pedibin and Hym-346, lower positional value thereby enhancing foot formation in hydra Development, January 2, 1999; 126(3): 517 - 524. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Bode The interstitial cell lineage of hydra: a stem cell system that arose early in evolution J. Cell Sci., January 6, 1996; 109(6): 1155 - 1164. [PDF]
|
|
|
|
|
|
I Scheurlen, S. Hoffmeister, and H. Schaller Presence and expression of G2 cyclins in the coelenterate hydra J. Cell Sci., January 5, 1996; 109(5): 1063 - 1069. [Abstract] [PDF]
|
|
|
|
|
|
A Grens, E Mason, J. Marsh, and H. Bode Evolutionary conservation of a cell fate specification gene: the Hydra achaete-scute homolog has proneural activity in Drosophila Development, January 12, 1995; 121(12): 4027 - 4035. [Abstract] [PDF]
|
|
|
|
|
|
X Zhang and M. Sarras Cell-extracellular matrix interactions under in vivo conditions during interstitial cell migration in Hydra vulgaris Development, January 2, 1994; 120(2): 425 - 432. [Abstract] [PDF]
|
|
