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Journal of Cell Science, Vol 16, 445-463, Copyright © 1974 by Company of Biologists

Submitted on March 13, 1974

Protoplast Regeneration from Normal and Bromodeoxyuridine-Resistant Sycamore Callus

S. W. J. BRIGHT 1 and D. H. NORTHCOTE 1

1 Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, England

Protoplasts have been prepared from normal and mutant lines of sycamore callus, maize root and tobacco leaf. Fusion was rare in sodium nitrate solutions. A mutant tissue culture was selected for by its resistance to 10 µg/ml bromodeoxyuridine. The mutant is sensitive to the Hypoxanthine-Aminopterin-Thymidine (HAT) selective medium but has normal thymidine transport and thymidine kinase activity.

Submitted on March 13, 1974






© The Company of Biologists Ltd 1974