spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by CENTURY, T. J.
Right arrow Articles by HOROWITZ, S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by CENTURY, T. J.
Right arrow Articles by HOROWITZ, S. B.

Journal of Cell Science, Vol 16, 465-471, Copyright © 1974 by Company of Biologists

Submitted on March 11, 1974

Sodium Exchange in the Cytoplasm and Nucleus of Amphibian Oocytes

T. J. CENTURY 1 and S. B. HOROWITZ 2

1 Cell Biology Institute, Swiss Federal Institute of Technology, CH-8006, Zurich, Switzerland
2 Cell Physiology Laboratory, Department of Biology, Michigan Cancer Foundation, Detroit, Michigan 48201, U.S.A.

Oocytes of Rana pipiens were incubated in 22Na-Ringer's solution and 22Na-23Na exchange in the nucleus and cytoplasm followed by low-temperature microdissection. Cytoplasmic sodium consists of 2 kinetic fractions: the larger (88%) slowly exchanging (t1/2 ~ 2 days), and the smaller (12%) rapidly exchanging (t1/2 ~ 53 min). Nuclear sodium consists of a single fraction whose concentration is similar and whose rate constant is identical with that of the rapidly exchanging cytoplasmic fraction. Our findings are consistent with a model of compartmentalization in which rapidly exchanging sodium is nearly uniformly distributed in the water of the nucleus and cytoplasm and its exchange limited by movement through the cell surface. On the other hand, slowly exchanging sodium is restricted to the cytoplasm, sorbed to or sequestered in some as yet unidentified cytoplasmic constituent.

Note:

To whom requests for reprints should be sent.

Submitted on March 11, 1974






© The Company of Biologists Ltd 1974