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Journal of Cell Science, Vol 16, 593-601, Copyright © 1974 by Company of Biologists

Submitted on April 4, 1974

Mitochondrial DNA from 4-Cell Stages of Ascaris Lumbricoides

H. TOBLER 1 and C. GUT 1

1 Institute of Zoology, University of Freiburg, CH-1700 Freiburg, Switzerland

Mitochondrial DNA (mtDNA) has been isolated from 4-cell stages of Ascaris lumbricoides. This DNA amounts to about 40% of the total quantity of 4-cell-stage DNA. Its buoyant density in neutral CsCl gradients is 1.686 g cm-3. Electron microscopy of mtDNA demonstrated the presence of circular molecules with an average contour length of 4.64 µm. About 15% of these molecules are supercoiled, covalently closed circles, whereas some 2% consist of double-forked circular molecules. The form and size of these branched molecules suggest that they are replicative intermediates.

Submitted on April 4, 1974






© The Company of Biologists Ltd 1974