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Journal of Cell Science, Vol 18, Issue 1 113-121, Copyright © 1975 by Company of Biologists

JOURNAL ARTICLES

Drosophila cell fusion induced by wheat germ agglutinin

RM Rizki, TM Rizki and CA Andrews

The effects of wheat germ agglutinin on Drosophila embryonic cell lines growing on cover-glasses was examined by scanning electron microscopy. At low concentrations of the lectin (5-10 mug/ml), cells spread against the glass surface and fused to form syncytia. At high concentration, damage to the cell surface was evidenced as extensive membrane shrivelling and loss of surface microfilaments. Fusion also occurred under these conditions. There was some indication that the morphology of cells in division remains undisturbed by wheat germ agglutinin. The coalescence of cells and morphologic disotrtion induced by wheat germ agglutinin were not inhibited by N-acetylglucosamine, the hapten inhibitor of the lectin, under the conditions utilized in this study.


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T. Rizki and R. Rizki
Blood cell surface changes in Drosophila mutants with melanotic tumors
Science, April 1, 1983; 220(4592): 73 - 75.
[Abstract] [PDF]


© The Company of Biologists Ltd 1975