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Journal of Cell Science, Vol 19, Issue 1 127-139, Copyright © 1975 by Company of Biologists

JOURNAL ARTICLES

Nucleocytoplasmic transport and distribution of an amino acid, in situ

M Frank and SB Horowitz

Ultra-low temperature techniques (microdissection and autoradiography) were used to study the nucleocytoplasmic distribution and transport of alpha-aminoisobutyric acid (AIB) in an amino acid-accumulating cell. In amphibiam oocytes incubated in AIB, the nuclear concentration of this non-metabolizable amino acid exceeds the cytoplasmic concentration by 45%, remaining constant both over time and variation in substrate concentration. The kinetics of uptake suggest that this nucleo-cytoplasmic asymmetry arises from solubility differences between the 2 compartments, and that the nuclear envelope plays a negligible role in amino acid transport. A solute exclusion model is offered to explain the nucleocytoplasmic asymmetry.




© The Company of Biologists Ltd 1975