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Journal of Cell Science, Vol 21, Issue 2 303-313, Copyright © 1976 by Company of Biologists

JOURNAL ARTICLES

The actions of restriction endonucleases on lampbrush chromosomes

DC Gould, HG Callan and CA Thomas

Lampbrush chromosomes from oocytes of Notophthalmus viridescens were dispersed in media containing restriction endonucleases isolated from Haemophilus and E. coli. These endonucleases cleave duplex DNAs at specific palindromic sequences of nucleotides, and several sensitive sites occur per micron of DNA. The overwhelming majority of the lateral loops of lampbrush chromosomes are extensively fragmented by these endonucleases, but an occasional pair of loops is refractory. A notable example of loops showing this refractory property are the giant loops on chromosome II in the presence of Hae. These loops, whose DNA-containing axes are several hundred micra long, are sensitive to other nucleases such as EcoB, endonuclease I and pancreatic DNase I; their refractory behavior towards Hae therefore indicates that the sequence sensitive to this particular endonuclease is systematically absent. This anomalous property can be comprehended if it be assumed that the axial DNA of the giant loops consists of tandem repeats of a sequence which happens not to include the sensitive site.


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 Mol. Biol. CellHome page
L. H. Pope, C. Xiong, and J. F. Marko
Proteolysis of Mitotic Chromosomes Induces Gradual and Anisotropic Decondensation Correlated with a Reduction of Elastic Modulus and Structural Sensitivity to Rarely Cutting Restriction Enzymes
Mol. Biol. Cell, January 1, 2006; 17(1): 104 - 113.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1976