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Journal of Cell Science, Vol 21, Issue 2 423-435, Copyright © 1976 by Company of Biologists

JOURNAL ARTICLES

Pollen-wall proteins: quantitative cytochemistry of the origins of intine and exine enzymes in Brassica oleracea

HI Vithanage and RB Knox

Simultaneous coupling methods for detection of acid phosphatase and non-specific esterase produce a coloured reaction product that is quantitatively related to enzyme content in freeze-sectioned Brassica pollen and tapetal cells. The intine-located acid phosphatase has 2 periods of synthesis: the first in late vacuolate period, associated with the completion of deposition of the intine polysaccharides; the second during pollen maturation, apparently reflecting cytoplasmic synthesis, Esterase activity accumulates in the tapetal cells until dissolution at early maturation period, when there is a dramatic rise in pollen-wall esterase activity, reflecting the transfer from tapetum to exine cavities. These quantitative studies confirm the gametophytic and sporophytic origins of the intine and exine proteins.




© The Company of Biologists Ltd 1976