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Journal of Cell Science, Vol 22, Issue 2 303-324, Copyright © 1976 by Company of Biologists

JOURNAL ARTICLES

Characterization of nuclear structures containing superhelical DNA

PR Cook, IA Brazell and E Jost

Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The concentration of salt present during isolation of human nucleoids affects their protein content. When made in I-95 M NaCl they lack histones and most of the proteins characteristic of chromatin; in 1-0 M NaCl they contain variable amounts of histones. The effects of various treatments on nucleoid integrity were investigated.


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© The Company of Biologists Ltd 1976