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Journal of Cell Science, Vol 26, Issue 1 359-371, Copyright © 1977 by Company of Biologists

JOURNAL ARTICLES

5-Bromodeoxyuridine-induced formation of virus-like particles in Naegleria gruberi EGs

FL Schuster and JS Clemente

Exposure of axenic cultures of the amoeboflagellate Naegleria gruberi EGs to the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU) resulted in the induction of virus-like particles (VLP) and various structures associated with their development and presumed transmission. Previously, VLP induction could be accomplished only by growing amoebae in the presence of living bacteria as a food source. Addition of excess thymidine along with BrdU did not block induction of particles. This account demonstrates that the EGs-VLP system responded to BrdU as do a number of mammalian cell lines harbouring latent viruses, and provides the basis for future work on the infectivity of the VLPs for other amoebae as well as tissue culture cells.




© The Company of Biologists Ltd 1977