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Journal of Cell Science, Vol 27, Issue 1 13-21, Copyright © 1977 by Company of Biologists

JOURNAL ARTICLES

The fixation of nuclei in glutaraldehyde

RJ Skaer and S Whytock

The production of an artifactual network in the nuclear sap of the salivary glands of Drosophila has been investigated. Mechanical stress to the cells, 3% glutaraldehyde containing more than 4 mM calcium, tannins, or cacodylate buffer or whose temperature is above 10 degrees C all enhance this artifactual effect. Over the range pH 6.65-7.4 there is no significant effect of pH. The inclusion of sucrose, and adenine nucleotides or other 'chelating' agents in the fixative reduces the effect, especially at low temperature (6-10 degrees C). These influences are additive and can abolish the artifactual effect. So too can 8% glutaraldehyde, but this is irrespective of temperature.


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A. Hassan and P. Cook
Visualization of replication sites in unfixed human cells
J. Cell Sci., January 6, 1993; 105(2): 541 - 550.
[Abstract] [PDF]


© The Company of Biologists Ltd 1977