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Journal of Cell Science, Vol 29, Issue 1 171-188, Copyright © 1978 by Company of Biologists

JOURNAL ARTICLES

Immunofluorescent anti-tubulin staining of spindles during meiotic maturation of mouse oocytes in vitro

PM Wassarman and K Fujiwara

Immunofluorescent anti-tubulin staining has been used to follow nuclear progression from dictyate to metaphase II during meiotic maturation of mouse oocytes in vitro. Antibody directed against tubulin isolated from sea-urchin eggs decorates the metaphase I and metaphase II spindles, as well as the cytoplasmic bridge, midbody, and polar body of the maturing mouse oocytes. Changes in the tubulin-specific staining pattern during meiotic maturation in vitro take place in a highly reproducible manner. Oocytes exposed continuously to cytochalasin B arrest at metaphase I and display a spindle which by immunofluorescent staining is virtually indistinguishable from the spindle of untreated oocytes.


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© The Company of Biologists Ltd 1978