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Journal of Cell Science, Vol 3, 381-389, Copyright © 1968 by Company of Biologists

Submitted on June 7, 1967
Revised on December 21, 1967

Studies on the Incorporation of DNA into Paramecium Aurelia

I. GIBSON 1

1 School of Biological Sciences, University of East Anglia, Norwich, Norfolk

Attempts have been made to transform the cell Paramecium aurelia with DNA preparations. No success was achieved for the genes concerned with maintenance of killer particles or surface antigens. Using radioautography, density gradient ultracentrifugation and nucleic acid hybridization it was shown that extracellular DNA was taken up by paramecia and remained as long nucleotide sequences. Possible reasons for the failure of transformation in the face of such observations are given.

Submitted on June 7, 1967
Revised on December 21, 1967






© The Company of Biologists Ltd 1968