spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by ORD, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ORD, M. J.

Journal of Cell Science, Vol 3, 483-491, Copyright © 1968 by Company of Biologists

Revised on January 19, 1968

The Synthesis of DNA Through the Cell Cycle of Amoeba Proteus

M. J. ORD 1

1 Department of Zoology, The University, Southampton, England

DNA synthesis has been investigated in Amoeba proteus by pulse-labelling cells of known age with [3H]thymidine. Ninety per cent of the DNA so labelled was synthesized during the first quarter of the cell cycle. Synthesis was in two peaks: the first occurring between 0.5 and 4 h after division and the second at about 10 h. All cells labelled at both peaks. The authenticity of the second peak was proved statistically.

Considerable variation was observed among amoebae of similar age. In experiments in which daughter amoebae underwent different treatments, differences in the rates of incorporation of [3H]thymidine due to differences in the nutrition of the cells were found to be a predominant cause of variation. Heavy feeding reduced labelling; starvation increased labelling while at the same time reducing variability.

Note:

Member of the Toxicology Research Unit, Medical Research Council Laboratories, Carshalton,Surrey.

Revised on January 19, 1968




This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
L. W. Parfrey, D. J. G. Lahr, and L. A. Katz
The Dynamic Nature of Eukaryotic Genomes
Mol. Biol. Evol., April 1, 2008; 25(4): 787 - 794.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1968