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Journal of Cell Science, Vol 38, Issue 1 181-191, Copyright © 1979 by Company of Biologists

JOURNAL ARTICLES

Ammonia plus another factor are necessary for differentiation in submerged clumps of Dictyostelium

J Sternfeld and CN David

Differentiation of Dictyostelium amoebae can occur in submerged clumps of cells; under an oxygen atmosphere mature stalk cells and spores form, as has been shown in previous work. This report shows that at least 2 factors are released by the cells under these conditions, and that both, together, are required for differentiation of stalk cells and spores. One of the factors is ammonia (NH3 + NH4+). The other factor(s) is heat stable and dialysable but has not yet been further characterized. The factors can be collected in conditioned medium and, when added to cells, stimulate differentiation. Conditioned medium loses its biological activity upon the removal of the NH3 + NH4+. When NH3 + NH4+ is added back, activity is restored. Because NH3 + NH4+, alone, has no activity, a second factor(s) in the conditioned medium must be required for differentiation. It is also shown that calcium inhibits differentiation in submerged clumps and that in calcium-free medium the timing of differentiation is essentially the same as under aerial conditions.
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R. Gomer and R. Firtel
Cell-autonomous determination of cell-type choice in Dictyostelium development by cell-cycle phase
Science, August 14, 1987; 237(4816): 758 - 762.
[Abstract] [PDF]


© The Company of Biologists Ltd 1979