spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yasin, R.
Right arrow Articles by Thompson, E. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yasin, R.
Right arrow Articles by Thompson, E. J.

Journal of Cell Science, Vol 38, Issue 1 201-210, Copyright © 1979 by Company of Biologists

JOURNAL ARTICLES

An abnormality of cell behaviour in human dystrophic muscle cultures: a time-lapse study

R Yasin, G Van Beers, PN Riddle, D Brown, G Widdowson and EJ Thompson

The initiation of monolayer mass cell cultures from adult human biopsies has revealed a striking abnormality in the growth and development pattern of muscle cultures from Duchenne-type dystrophy. This abnormality in cell behaviour was seen as early as 4 days in culture, well before myotube formation or confluence, and consisted of areas where cells clustered together in a multilayered mass rather than showing the typical monolayer distribution normally observed. To gain some insight into the mechanism of cell cluster development, we have examined such a culture by time-lapse cinematography and also the cell behaviour of other control cultures. The results of this study show that the clusters enlarged primarily by cell division and, to a lesser extent, by the acquisition of neighbouring cells. Furthermore, none of the single cells surrounding the clusters exhibited contact inhibition of movement. This behaviour was not observed in the other cultures examined. These findings could be indicative of an abnormality in the cell surface or cell-locomotory machinery of dystrophic cells.




© The Company of Biologists Ltd 1979