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Journal of Cell Science, Vol 39, 247-256, Copyright © 1979 by Company of Biologists

Submitted on February 27, 1979

Isolation of a Residual Protein Structure from Nuclei of the Myxomycete Physarum Polycephalum

K. R. MITCHELSON 1, A. G. M. BEKERS 2, and F. WANKA 2

1 Department of Chemical Cytology, University of Nijmegen, Toernooiveld, Nijmegen, The Netherlands; Biophysics Laboratory, St Michael's Building, White Swan Road, Portsmouth, England
2 Department of Chemical Cytology, University of Nijmegen, Toernooiveld, Nijmegen, The Netherlands

A nuclear framework structure has been obtained from isolated interphase nuclei of Physarum polycephalum by extraction with 2.5 M NaCl and subsequent digestion with DNase. Whole-mount electron micrographs showed a nuclear lamina containing residual pore structures associated with the fibrous internal matrix. The matrix was continuous with fibrillar remnants of the nucleolus. The structure was shown to consist of 2 major polypeptides of 23000 and 36500 Daltons as well as 30 to 40 minor polypeptides of various molecular weight classes. The 2 major polypeptides were also prominent in preparations of the residual nucleolar material, suggesting that matrix proteins are common to both structures. The predominance of low-molecular-weight polypeptides in Physarum nuclear matrix suggests that there may be significant differences in composition of nuclear structural proteins between lower and higher eukaryotes.

Submitted on February 27, 1979




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© The Company of Biologists Ltd 1979