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Journal of Cell Science, Vol 39, Issue 1 53-62, Copyright © 1979 by Company of Biologists

JOURNAL ARTICLES

Electron-microscopy of intact nuclear DNA from human cells

SJ McCready, A Akrigg and PR Cook

Structures retaining many of the morphological features of nuclei may be released by lysing human cells in a non-ionic detergent and 2 M NaCl. Such nucleoids contain all the nuclear DNA packaged within a flexible cage of RNA and protein. HeLa nucleoids have been spread at an air-water interface and viewed in the electron microscope. A tangled network of superhelical fibres surrounds the collapsed cage. Irradiation with gamma-rays abolishes supercoiling and treatment with the untwisting enzyme or a low concentration of ethidium reduces it. A high concentration of ethidium induces supertwisting. The nuclear DNA of higher cells can be isolated naked, supercoiled and intact.


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© The Company of Biologists Ltd 1979