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Journal of Cell Science, Vol 44, Issue 1 123-133, Copyright © 1980 by Company of Biologists

JOURNAL ARTICLES

Microtubule-organizing centres in binucleate cells and homosynkaryons

FM Watt, E Sidebottom and H Harris

Immunofluorescence studies showed that most binucleate Vero cells formed by virus-induced fusion or by inhibition of cytokinesis had a single microtubule-organizing centre (MTOC) when examined during the reassembly of microtubules after chilling, but two or more organizing centres when examined after exposure to colcemid. These findings suggest that although binucleate cells initially contain more MTOC than mononucleate cells, the extra MTOC are normally aggregated, so that the number of MTOC in binucleate cells tends to be reduced very quickly to that in mononucleate cells.




© The Company of Biologists Ltd 1980