spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Allen, R. D.
Right arrow Articles by Fok, A. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Allen, R. D.
Right arrow Articles by Fok, A. K.

Journal of Cell Science, Vol 45, Issue 1 131-145, Copyright © 1980 by Company of Biologists

JOURNAL ARTICLES

Membrane recycling and endocytosis in Paramecium confirmed by horseradish peroxidase pulse-chase studies

RD Allen and AK Fok

Paramecium caudatum cells were pulse-chased in horseradish peroxidase (HRP) using pulses of 30 s to follow endosome (endocytotic vesicle) formation from defaecating digestive vacuoles and 3-min pulses to follow the movement and fate of these vesicles in the cell. Endosomes formed during the 30 s in HRP are heavily labelled with HRP reaction product and are mostly flattened. Some align along microtubules that point toward the cytopharynx. Diskoidal vesicles at the cytopharynx are unlabelled in unchased cells. Cells exposed to a 3-min HRP pulse contain varied amounts of HRP-labelled diskoidal vesicles at the cytopharynx but no labelled vesicles near their closed cytoprocts. Labelled vesicles are also found aligned along the cytopharyngeal microtubular ribbons. Diskoidal vesicles are no longer labelled after a 47-min chase. Use of pulse-chase HRP cytochemistry supports the hypothesis that the membrane of digestive vacuoles retrieved at the cytoproct is moved along microtubular ribbons directly to the cytopharynx where the membrane enters the diskoidal vesicle pool. This membrane appears to enter neither the Golgi nor lysosomal systems in its passage. HRP reaction product is also found in vesicles near the parasomal sacs, in some secondary lysosomes and in small spherical vesicles that are probably trichocyst membrane fragments. Possibly some membrane from parasomal sacs or condensing digestive vacuoles may also enter the diskoidal vesicle pool but apparently not membrane from trichocysts.


This article has been cited by other articles:


Home page
 J. Exp. Biol.Home page
H. K. Gronlien, T. Berg, and A. M. Lovlie
In the polymorphic ciliate Tetrahymena vorax, the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes
J. Exp. Biol., July 15, 2002; 205(14): 2089 - 2097.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. Yamauchi, M. S. Aihara, M. Ishida, R. D. Allen, and A. K. Fok
Cloning and Sequencing of a Protein Involved in Phagosomal Membrane Fusion in Paramecium
Mol. Biol. Cell, April 1, 1999; 10(4): 1031 - 1041.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
R. Allen, N. Bala, R. Ali, D. Nishida, M. Aihara, M Ishida, and A. Fok
Rapid bulk replacement of acceptor membrane by donor membrane during phagosome to phagoacidosome transformation in Paramecium
J. Cell Sci., January 3, 1995; 108(3): 1263 - 1274.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
R. Allen, L Ma, and A. Fok
Acidosomes: recipients of multiple sources of membrane and cargo during development and maturation
J. Cell Sci., January 9, 1993; 106(1): 411 - 422.
[Abstract] [PDF]


© The Company of Biologists Ltd 1980