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Journal of Cell Science, Vol 5, 727-743, Copyright © 1969 by Company of Biologists

Submitted on February 24, 1969

Isopropyl N-Phenylcarbamate Affects Spindle Microtubule Orientation in Dividing Endosperm Cells of Haemanthus Katherinae Baker

P. K. HEPLER 1 and W. T. JACKSON 2

1 The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138, U.S.A.
2 The Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, U.S.A.

The mitotic inhibitor isopropyl N-phenylcarbamate (IPC) effects changes in the ultra-structure of the dividing endosperm cells of Haemanthus katherinae. In cells treated with 10 p.p.m. IPC for 0.5-2 h the microtubules lose their parallel alignment and become oriented in radial arrays. These radial arrays are interpreted as micropoles of the spindle apparatus and are thought to be the focal points for the chromosomes as they aggregate into micronuclei. Thus, IPC has a markedly different effect on the structure of the spindle apparatus than colchicine, which is observed at concentrations of 50 p.p.m. for 1 h to destroy the microtubules in Haemanthus. The structure of the microtubules appears unaffected by IPC since those from control and treated cells are similar in diameter (22 nm), in degree of staining, and in sensitivity to the fixation. In addition, the chromosomes and cytoplasmic components such as mitochondria, plastids, dictyosomes, elements of the endoplasmic reticulum, and ribosomes are not structurally modified by treatment with IPC.

Submitted on February 24, 1969






© The Company of Biologists Ltd 1969