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Journal of Cell Science, Vol 6, 299-321, Copyright © 1970 by Company of Biologists

Submitted on May 20, 1969

The Structure of Sycamore Callus Cells During Division in a Partially Synchronized Suspension Culture

K. ROBERTS 1 and D. H. NORTHCOTE 1

1 Department of Biochemistry, University of Cambridge, Cambridge, England

Sycamore suspension callus cells have been partially synchronized to give a culture with a mitotic index of 15%. Living dividing cells of the culture have been examined with Nomarski differential interference optics and a comparable study made on fixed cells with the electron microscope.

An organized band of reticulate cytoplasm partially encircles the nucleus at mitosis. The cell divides by the formation of a phragmosome which grows across the large vacuole; this allows the organization of the cytoplasm which forms the cell plate to be examined separately from the more general cytoplasm of the cell. The cell plate grows from one side of the cell to the other and down its length a complete developmental sequence can be seen.

The Golgi bodies and the endoplasmic reticulum are probably involved in the formation of material for the construction of the cell plate and young cell wall. Microfibrils are formed within the plate in the more mature regions, while material contained within vesicles is incorporated at the young growing edge. At the edge of the plate microtubules are found and these correspond to the fibrillar appearance of the phragmoplast seen with the optical microscope.

In the living cell an active movement of organelles along the peripheral cytoplasm can be seen and with fixed cells viewed with the electron microscope microtubules are often found adjacent to the plasmalemma and lying close to mitochondria, crystal-containing bodies and plastids.

The appearance of crystal-containing bodies and plastids containing phytoferritin is described.

Submitted on May 20, 1969




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© The Company of Biologists Ltd 1970