spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, B. D.
Right arrow Articles by Lin, H. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, B. D.
Right arrow Articles by Lin, H. S.

Journal of Cell Science, Vol 70, Issue 1 147-166, Copyright © 1984 by Company of Biologists

JOURNAL ARTICLES

Receptor-mediated binding and internalization of colony-stimulating factor (CSF-1) by mouse peritoneal exudate macrophages

BD Chen, C Kuhn and HS Lin

Colony-stimulating factor (CSF-1) purified from L-cell-conditioned medium is a haemopoietic growth factor that specifically stimulates the proliferation and differentiation of mononuclear phagocytes. Using radioactively labelled CSF-1 [( 125I]CSF-1), the presence of specific CSF-1 receptor has been identified in the cells of the mononuclear phagocytic series and their precursors only. To determine the fate of [125I]CSF-1 bound to peritoneal exudate macrophages (PEM) at 37 degrees C, we have examined the distribution of radioactivity as a function of time by quantitative electron microscopic autoradiography. At 0 degrees C, we have localized the initial step in the binding of [125I]CSF-1 to the plasma membrane and its invaginations of the mouse PEM. Approximately 16% of the macrophages were not labelled at this time point. When the temperature was raised to 37 degrees C, the labelled CSF-1 was internalized progressively by the cells in a time-dependent fashion. The proportion of grains associated with the phagolysosome compartment increased progressively, reaching a plateau by 40 min after warming up, while the relative areas of the surface membrane and its invaginations decreased in invaginated membrane. At 37 degrees C, incubation with unlabelled CSF-1 resulted in a "down-regulation' of the subsequent [125I]CSF-1-binding activity by PEM in a time- and dose-dependent fashion. The restoration of CSF-1-binding activity after CSF-1 induced down-regulation was inhibited by cycloheximide, a potent protein synthesis inhibitor. These data provide direct evidence that at 37 degrees C, saturable binding of CSF-1 to PEM is followed by internalization and cellular degradation of the ligand and possibly its receptor by phagolysosomes.


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
V. Kanagasundaram, A. Jaworowski, R. Byrne, and J. A. Hamilton
Separation and Characterization of the Activated Pool of Colony-Stimulating Factor 1 Receptor Forming Distinct Multimeric Complexes with Signalling Molecules in Macrophages
Mol. Cell. Biol., June 1, 1999; 19(6): 4079 - 4092.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1984