spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smith, G. P.
Right arrow Articles by Peters, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smith, G. P.
Right arrow Articles by Peters, T. J.

Journal of Cell Science, Vol 76, Issue 1 167-178, Copyright © 1985 by Company of Biologists

JOURNAL ARTICLES

Isolation and characterization of alkaline phosphatase-containing granules (phosphasomes) from human polymorphonuclear leucocytes

GP Smith, G Sharp and TJ Peters

Human polymorphonuclear leucocytes were homogenized in isotonic sucrose and subjected to analytical subcellular fractionation by centrifugation on a discontinuous sucrose density gradient to produce a phosphasome-enriched fraction, contaminated primarily by plasma membrane. Experiments to separate these membrane fractions by centrifugation on gradients of Nycodenz or Percoll, or by toroidal coil counter current chromatography, were unsuccessful. Fractionation carried out on neutrophils previously suspended in isotonic sucrose containing a low concentration of digitonin resulted in the preparation of a highly purified phosphasome fraction, free of plasma membrane components. Electron-microscope cytochemistry of the purified fraction identified the phosphasomes as regular and irregular-shaped spheres and rods, the alkaline phosphatase being associated with the inner aspect of the vesicle membrane. These granule structures were very similar to phosphasomes observed in intact neutrophils. A proportion of the endoplasmic reticulum and Golgi membrane marker enzymes remained associated with the phosphasomes throughout the separation procedures.


This article has been cited by other articles:


Home page
 BloodHome page
B. Martin-Martin, S. M. Nabokina, J. Blasi, P. A. Lazo, and F. Mollinedo
Involvement of SNAP-23 and syntaxin 6 in human neutrophil exocytosis
Blood, October 1, 2000; 96(7): 2574 - 2583.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
N Borregaard, L. Miller, and T. Springer
Chemoattractant-regulated mobilization of a novel intracellular compartment in human neutrophils
Science, September 4, 1987; 237(4819): 1204 - 1206.
[Abstract] [PDF]


© The Company of Biologists Ltd 1985