spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gowing, L. R.
Right arrow Articles by Banyard, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gowing, L. R.
Right arrow Articles by Banyard, M. R.

Journal of Cell Science, Vol 76, Issue 1 255-267, Copyright © 1985 by Company of Biologists

JOURNAL ARTICLES

Actin synthesis in tumorigenic and non-tumorigenic human hybrid cells

LR Gowing, RL Tellam and MR Banyard

We have previously shown that the total actin content of tumorigenic HeLa/fibroblast somatic cell hybrids is significantly lower than that of the non-tumorigenic hybrid cells. A measure of actin synthesis, relative to total protein synthesis, was obtained for these cells to determine whether the reduced actin content of the tumorigenic cells is due to specific suppression of actin synthesis. Actin synthesis was measured in cells labelled with [35S]methionine using DNase I affinity chromatography to isolate the actin quantitatively. The results show that actin synthesis is not specifically suppressed, since the relative amount of actin synthesized is constant for the tumorigenic and non-tumorigenic cell lines. The reduced actin content of the tumorigenic cells is therefore most likely to be the result of increased degradation of actin.




© The Company of Biologists Ltd 1985