spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Crossley, R.
Right arrow Articles by Holberton, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Crossley, R.
Right arrow Articles by Holberton, D.

Journal of Cell Science, Vol 78, Issue 1 205-231, Copyright © 1985 by Company of Biologists

JOURNAL ARTICLES

Assembly of 2.5 nm filaments from giardin, a protein associated with cytoskeletal microtubules in Giardia

R Crossley and D Holberton

The giardins are a family of approximately 30000 Mr structural proteins found in microribbons attached to microtubules in the disc cytoskeleton of Giardia. After examining the solubility of giardins in various agents, a method has been developed to extract these polypeptides and subsequently precipitate them selectively. The giardin chains are soluble in 10 mM-HEPES/EDTA buffer at high pH and low ionic strength, but become insoluble in 10 mM-MES/EDTA buffer at pH 6.7 when the ionic strength is raised above 50 mM salt. By dialysing giardin extracts in turn against dissociating and reassembly buffers, the purification is obtained of a subset of giardin chains identified by sodium dodecyl sulphate/polyacrylamide gel electrophoresis as the cytoskeleton bands 14a, 14b and 15. The structures forming under assembly conditions are all composed of fine filaments, 2-3 nm in diameter. Filaments after the first cycle of assembly are found in bundles, narrow ribbons of two or three filaments, and large ordered tactoids. Assembly after a second cycle of solubilization yields a more uniform population of long ribbons. Both the tactoids and the second cycle ribbons are transversely banded at the 15 nm interval characteristic of microribbons in the cytoskeleton. Filaments in the tactoids are precisely placed at a centre-to-centre separation of 2.5 nm. Other structural features of the tactoids are discussed in relation to the association behaviour and possible dimensions of the giardin molecular subunit.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Szkodowska, M. C. M. Muller, C. Linke, and H. Scholze
Annexin XXI (ANX21) of Giardia lamblia Has Sequence Motifs Uniquely Shared by Giardial Annexins and Is Specifically Localized in the Flagella
J. Biol. Chem., July 5, 2002; 277(28): 25703 - 25706.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
G. Faubert
Immune Response to Giardia duodenalis
Clin. Microbiol. Rev., January 1, 2000; 13(1): 35 - 54.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1985