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Journal of Cell Science, Vol 8, 693-700, Copyright © 1971 by Company of Biologists

Submitted on September 24, 1970

The Quantitative Glucose and Mineral Nutrient Requirements of Mouse LS (Suspension) Cells in Chemically Defined Medium

J. R. BIRCH 1 and S. J. PIRT 2

1 Department of Microbiology, Queen Elizabeth College (University of London), Campden Hill, London, W. 8, England; Tate and Lyle Ltd., Research Centre, ‘Ravensbourne’, Westerham Road, Keston, Kent, England
2 Department of Microbiology, Queen Elizabeth College (University of London), Campden Hill, London, W. 8, England

The influences of glucose, phosphate, magnesium and potassium concentration on growth rate and maximum population density were studied. From the data obtained the growth yields for glucose and phosphorus were found to be 2.4 x 105 cells (i.e. 0.76 mg dry weight)/mg glucose and 2.2 x 105 (i.e. 69 µg dry weight)/µg P. The growth response to magnesium and potassium was complex since there were threshold concentrations of these metals below which no growth occurred. The growth yield for potassium (2.4 x 105 cells (i.e. 75 µg dry weight)/µg K) was obtained by measuring potassium uptake during growth. Omission of calcium ions from the medium resulted in poor reproducibility of growth.

Submitted on September 24, 1970






© The Company of Biologists Ltd 1971