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Journal of Cell Science, Vol 81, Issue 1 105-123, Copyright © 1986 by Company of Biologists
JOURNAL ARTICLES |
RM Hembry, G Murphy, TE Cawston, JT Dingle and JJ Reynolds
A specific antiserum to rabbit bone collagenase was raised in a sheep and shown to react with collagenase from several mammalian species. This antiserum was used to demonstrate that only active collagenase binds to and can be immunolocalized on collagen fibrils and, by indirect immunofluorescence, the secretion of latent collagenase by stimulated rabbit chondrocytes and cells of the post-partum involuting rabbit uterus. The ionophore monensin was used to demonstrate intracellular accumulation of collagenase in the Golgi apparatus of both stimulated chondrocytes and involuting uterine cells. Collagenase was not detectable in either normal cartilage or non-gravid uterus. These results are discussed in relation to other studies of collagenase immunolocalization reported in the literature.
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  R. M. Hembry, J. Dyce, I. Driesang, E. B. Hunziker, A. J. Fosang, J. A. Tyler, and G. Murphy Immunolocalization of Matrix Metalloproteinases in Partial-Thickness Defects in Pig Articular Cartilage : A Preliminary Report J. Bone Joint Surg. Am., June 1, 2001; 83(6): 826 - 838. [Abstract] [Full Text]
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 I Kokorine, E Marbaix, P Henriet, Y Okada, J Donnez, Y Eeckhout, and P. Courtoy Focal cellular origin and regulation of interstitial collagenase (matrix metalloproteinase-1) are related to menstrual breakdown in the human endometrium J. Cell Sci., January 8, 1996; 109(8): 2151 - 2160. [Abstract] [PDF]
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K Fuller and T. Chambers Localisation of mRNA for collagenase in osteocytic, bone surface and chondrocytic cells but not osteoclasts J. Cell Sci., January 6, 1995; 108(6): 2221 - 2230. [Abstract] [PDF]
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 J.J. Reynolds, R.M. Hembry, and M.C. Meikle Connective Tissue Degradation in Health and Periodontal Disease and the Roles of Matrix Metalloproteinases and Their Natural Inhibitors Advances in Dental Research, July 1, 1994; 8(2): 312 - 319. [Abstract] [PDF]
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P. Hill, G Murphy, A. Docherty, R. Hembry, T. Millican, J. Reynolds, and M. Meikle The effects of selective inhibitors of matrix metalloproteinases (MMPs) on bone resorption and the identification of MMPs and TIMP-1 in isolated osteoclasts J. Cell Sci., January 11, 1994; 107(11): 3055 - 3064. [Abstract] [PDF]
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E van der Zee, V Everts, K Hoeben, and W Beertsen Immunolocalisation of collagenase in rabbit periosteal tissue explants and extraction of the enzyme. The effect of the cytokines IL-1 alpha and EGF J. Cell Sci., January 4, 1994; 107(4): 1047 - 1053. [Abstract] [PDF]
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M. C. Meikle, S. Bord, R. M. Hembry, J. Compston, P. I. Croucher, and J. J. Reynolds Human osteoblasts in culture synthesize collagenase and other matrix metalloproteinases in response to osteotropic hormones and cytokines J. Cell Sci., December 1, 1992; 103(4): 1093 - 1099. [Abstract] [PDF]
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 C A Brenner, R R Adler, D A Rappolee, R A Pedersen, and Z Werb Genes for extracellular-matrix-degrading metalloproteinases and their inhibitor, TIMP, are expressed during early mammalian development. Genes & Dev., June 1, 1989; 3(6): 848 - 859. [Abstract] [PDF]
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