spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by HESLOP-HARRISON, J.
Right arrow Articles by CIAMPOLINI, F.
Right arrow Search for Related Content
PubMed
Right arrow Articles by HESLOP-HARRISON, J.
Right arrow Articles by CIAMPOLINI, F.

Journal of Cell Science, Vol 86, 1-8, Copyright © 1986 by Company of Biologists

Submitted on May 28, 1986
Accepted on June 27, 1986

Actin During Pollen Germination

J. HESLOP-HARRISON 1, Y. HESLOP-HARRISON 1, M. CRESTI 2, A. TIEZZI 2, and F. CIAMPOLINI 2

1 Cell Physiology Laboratory, Welsh Plant Breeding Station, University College of Wales, Plas Gogerddan, Aberystwyth SY23 3EB, UK
2 Dipartimento di Biologia Ambientale, Universita di Siena Via P. A. Mattioli, 53100 Siena, Italy

The cytoplasm of the vegetative cell of the ungerminated pollen grain of Endymton non-scriplus and other angiosperm species contains numerous fusiform bodies sometimes exceeding 15µm in length and 2.5 µm in width, which bind fluorescent-labelled phalloidin and are likely therefore to constitute a storage form of actin. The bodies are dispersed during the activation of the pollen, being replaced by aggregates of slender phalloidin-binding fibrils, which converge towards the germination apertures and are present in the emerging pollen tube. The storage bodies appear to be homologous with crystalline-fibrillar structures, shown in an earlier paper to be abundantly present in the vegetative cells of Nicotiana pollen. These are composed of massive aggregates of linearly disposed units with individual widths of 4-7 nm, probably to be interpreted as actin microfilaments.

Vegetative-cell protoplasts from mature but ungerminated pollen disrupted in osmotically balancing medium release extended phalloidin-binding fibrils of a kind not observed in the intact grain. It is suggested that these are derived by the rapid dissociation of the compact actin storage bodies present in the vegetative cell at this stage of development.

Key words: pollen actin, pollen germination, Helleborus, Narcissus, Endymion, Hordeum

Submitted on May 28, 1986
Accepted on June 27, 1986




This article has been cited by other articles:


Home page
 Plant CellHome page
J.-L. Qiu, R. Jilk, M. D. Marks, and D. B. Szymanski
The Arabidopsis SPIKE1 Gene Is Required for Normal Cell Shape Control and Tissue Development
PLANT CELL, January 1, 2002; 14(1): 101 - 118.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
E. Chytilova, J. Macas, E. Sliwinska, S. M. Rafelski, G. M. Lambert, and D. W. Galbraith
Nuclear Dynamics in Arabidopsis thaliana
Mol. Biol. Cell, August 1, 2000; 11(8): 2733 - 2741.
[Abstract] [Full Text]

Home page
 Plant CellHome page
D. B. Szymanski, M. D. Marks, and S. M. Wick
Organized F-Actin Is Essential for Normal Trichome Morphogenesis in Arabidopsis
PLANT CELL, December 1, 1999; 11(12): 2331 - 2348.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
E. Yokota and K.-i. T. a. T. Shimmen
Actin-Bundling Protein Isolated from Pollen Tubes of Lily . Biochemical and Immunocytochemical Characterization
Plant Physiology, April 1, 1998; 116(4): 1421 - 1429.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
J. Andersland and M. Parthasarathy
Conditions affecting depolymerization of actin in plant homogenates
J. Cell Sci., January 4, 1993; 104(4): 1273 - 1279.
[Abstract] [PDF]


© The Company of Biologists Ltd 1986