spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Behnel, H. J.
Right arrow Articles by Weckbart, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Behnel, H. J.
Right arrow Articles by Weckbart, G.

Journal of Cell Science, Vol 87, Issue 1 197-201, Copyright © 1987 by Company of Biologists

JOURNAL ARTICLES

Induced stabilization of the transmembrane potential of Drosophila cells by heat shock and periodic applications of chloramphenicol

HJ Behnel and G Weckbart
Institut fur Genetik, Universitat des Saarlandes, Saarbrucken, GFR.

The transmembrane potential of Drosophila salivary gland cells is largely decreased (by 78% within 120 min) in response to the application of 5 mM-chloramphenicol (CAP), with an initial slope of 0.5 mV min-1. This depolarization is reversed immediately after the CAP concentration is reduced to 0.06 mM by step-wise dilution with normal medium. At the same concentration, thiamphenicol (TAP) induces only a small reversible depolarization by less than 30% within 120 min. These results are in agreement with the different effects of CAP and TAP on respiration and induction of the heat-shock genes, as known from previous data. The extent of induced membrane depolarization decreases with the number of repeated applications of CAP to the same cell, alternating with 75-min periods of recovery. Moreover, reduced sensitivity to CAP is also observed in cells recovering from a transient heat shock (30 min, 36 degrees C) 45 min prior to the addition of CAP. This phenomenon is inhibited by cycloheximide (0.1 mM), which suggests an involvement of heat-induced proteins in the stabilization of certain membrane functions.




© The Company of Biologists Ltd 1987