spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Francis, G. W.
Right arrow Articles by Gingell, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Francis, G. W.
Right arrow Articles by Gingell, D.

Journal of Cell Science, Vol 87, Issue 4 519-523, Copyright © 1987 by Company of Biologists

JOURNAL ARTICLES

Direct measurement of cell detachment force on single cells using a new electromechanical method

GW Francis, LR Fisher, RA Gamble and D Gingell
CSIRO Division of Food Research, North Ryde, NSW Australia.

We describe a new device in which an accurately measured force is applied to individual adherent cells while the topography of the adhesion zone is simultaneously monitored. The force is applied via a flexible glass micropipette, attached by suction to the cell under study, and is calculated directly from the measured pipette deflection. Regions of close contact in the adhesion zone are observed using interference reflection microscopy. We have used the device to measure the force required to detach human red blood cells from hydrophobic and hydrophilic glass surfaces, and to detach Dictyostelium discoideum amoebae from a hydrophobic glass surface. The measured forces per unit length of contact perimeter are within an order of magnitude of the tensions required for membrane rupture.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
P. H. Tsang, G. Li, Y. V. Brun, L. B. Freund, and J. X. Tang
Adhesion of single bacterial cells in the micronewton range
PNAS, April 11, 2006; 103(15): 5764 - 5768.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P Lamoureux, Z. Altun-Gultekin, C Lin, J. Wagner, and S. Heidemann
Rac is required for growth cone function but not neurite assembly
J. Cell Sci., January 3, 1997; 110(5): 635 - 641.
[Abstract] [PDF]


© The Company of Biologists Ltd 1987