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Journal of Cell Science, Vol 87, 595-607, Copyright © 1987 by Company of Biologists

Submitted on November 24, 1986
Accepted on March 3, 1987

Microtubules and cell wall development in differentiating protophloem sieve elements of Triticum aestivum L

E. P. ELEFTHERIOU 1

1 Department of Botany, University of Thessalomki GR 54006 Thessaloniki, Greece

The densities of microtubules (MTs) along the lateral walls of developing sieve elements in root protophloem of wheat have been investigated by electron microscopy. They increase gradually in the very young sieve elements to reach a maximum just before the initiation of wall thickening. During wall increment MTs remain at high densities (more than 10 MTs µm-1), but their number declines abruptly when wall material deposition ceases.

Cell wall thickening is not uniform: broad ridges alternate with narrow depressions, the latter occupied by plasmodesmata. During wall material deposition MTs overlie the thickenings only, being entirely absent from the non-thickened areas. The orientation of MTs reflects that of the currently deposited cellulose microfibrils in the cell wall, all being perpendicular to the direction of cell expansion. Numerous vesicles, apparently of Golgi apparatus origin, are encountered amongst the cortical arrays of MTs. Though the least spacing between the contiguous MTs is much smaller than the diameter of even the smallest vesicles, the latter were seen amongst the MTs, indicating that MTs do not prevent the vesicles from passing between them towards the developing area.

All results favour the suggestion that MTs in sieve elements are involved in cell wall pattern development, cellulose microfibril orientation, and presumably in cell elongation.

Key words: wheat, protophloem sieve elements, microtubules, cell wall thickenings

Submitted on November 24, 1986
Accepted on March 3, 1987






© The Company of Biologists Ltd 1987