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Journal of Cell Science, Vol 87, Issue 5 695-703, Copyright © 1987 by Company of Biologists

JOURNAL ARTICLES

Establishment of a tissue culture system for epithelial cells derived from human pancreas: a model for the study of cystic fibrosis

A Harris and L Coleman
Paediatric Research Unit, United Medical, School of Guy's, Hospital, London, UK.

A tissue culture system for epithelial cells derived from human foetal pancreas has been established. The cultured cells show many ultrastructural features of interlobular duct cells. Immunocytochemical and histochemical evidence is presented in support of the view that these cells are ductal in origin. They are likely to be one of the few cell types that express the basic defect of cystic fibrosis in vitro. The cells may be passaged and sufficient material obtained to permit biochemical and molecular biological analysis.


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J. Chambers and A Harris
Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells
J. Cell Sci., January 6, 1993; 105(2): 417 - 422.
[Abstract] [PDF]


© The Company of Biologists Ltd 1987