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Journal of Cell Science, Vol 88, 407-414, Copyright © 1987 by Company of Biologists

Submitted on February 11, 1987
Accepted on May 11, 1987

Observations on rRNA gene replication in Chara vulgaris evaluated by in situ [3H]rRNA-DNA hybridization

MARIA KWIATKOWSKA 1, A. K. KONONOWICZ 2, and KRYSTYNA JANAS 3

1 Laboratory of Cytophysiology
2 Department of Plant Cytology and Cytochemistry
3 Laboratory of Plant Growth Substances, Institute of Physiology and Cytology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland

In situ [3H]rRNA-DNA hybridization showed that: (1) during the cell cycle in antheridial filaments of Chara vulgaris the rDNA gene content doubles during a 2h period in mid-S-phase, which corresponds to about 13% of the duration of this phase; (2) doubling of the number of grains occurs during mid-S-phase irrespective the developmental stage of the antheridial filaments; (3) the numbers of autoradiographic grains in comparable phases of successive cell cycles preceding spermatid formation are the same. Thus the gradual reduction of total nucleolar volume (estimated per nucleus) occurring in successive cell cycles preceding spermatid formation is not associated with a decrease in the number of rDNA genes.

Key words: Chara vulgaris, ribosomal gene replication, cell cycle, antheridial filaments, [3H]rRNA-DNA hybridization

Submitted on February 11, 1987
Accepted on May 11, 1987




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A Majewska-Sawka and M. Rodriguez-Garcia
rRNA distribution during microspore development in anthers of Beta vulgaris L. quantitative in situ hybridization analysis
J. Cell Sci., January 4, 1996; 109(4): 859 - 866.
[Abstract] [PDF]


© The Company of Biologists Ltd 1987