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Journal of Cell Science, Vol 88, 553-562, Copyright © 1987 by Company of Biologists

Submitted on July 15, 1987
Accepted on September 10, 1987

Allelic antigen and membrane-anchor epitopes of Paramecium primaurelia surface antigens

YVONNE CAPDEVILLE 1, FRANCOIS CARON 1, CLAUDE ANTONY 1, CHRISTIANE DEREGNAUCOURT 1, and ANNE-MARIE KELLER 1

1 Département I du Centre de Génétique Moléculaire, Centre National die la Recherche Scientifique, 91190 Gif-sur-Yvette, France

Author for correspondence

Paramecium aurelia can express a repertoire of surface antigens (SAgs) according to culture conditions. These high Mr proteins are anchored in the plasma membrane by a glycolipid, and they can be isolated in two different forms, an amphiphilic membrane-bound form (mSAg) and a hydrophilic soluble form (sSAg). Endogenous or exogenous phospholipase C can convert mSAg to sSAg with unmasking of a carbohydrate antigenic determinant similar to that found in the soluble form of Trypanosoma variant surface glycoproteins and called the cross-reacting determinant. By immunizing mice with cilia from strain 156 of P. primaurelia expressing the G SAg, we obtained six monoclonal antibodies against the 156G SAg, which could be classified into two groups. Y4 and Y8, representative of each group, have been characterized by checking their reactivity in situ and in vivo towards a series of allelic G and D SAgs in P. primaurelia and the 51B SAg in P. tetraurelia.

The monoclonal Y4 recognizes a conformational determinant, accessible in vivo and common to all the G SAgs. Thus, Y4 defines a G locus-specific epitope that corresponds to a conserved region inside a polymorphic domain. The monoclonal Y8 recognizes two homologous determinants whose detection depends on the presence or absence of the SAg membrane-anchor, and which are mutually exclusive: one is found in the reduced soluble form of all the SAgs and other surface proteins, the cross-reacting glycoproteins (CRGs); the other occurs in the unreduced membrane-bound form of the G SAgs. Thus, Y8 enables us to demonstrate that the membrane-anchor of Paramecium SAgs contains an additional hidden determinant close to the cross-reacting determinant and to discriminate between the membrane-bound and soluble form of SAgs.

The in situ organization of the 156G SAg molecules is also discussed on the basis of immunogold labelling obtained using Y4 and a polyclonal antiserum.

Key words: surface antigen, membrane-anchor, monoclonal antibody, Paramecium

Submitted on July 15, 1987
Accepted on September 10, 1987




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[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1987