|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 89, Issue 3 287-296, Copyright © 1988 by Company of Biologists
JOURNAL ARTICLES |
A Slominski, G Moellmann, E Kuklinska, A Bomirski and J Pawelek
Department of Dermatology, Yale University School of Medicine, New Haven, CT 06510.
We describe results demonstrating the positive regulation of melanogenesis by two substrates of the melanogenic pathway. We have found that L-tyrosine and L-dihydroxyphenylalanine (L-dopa), whose metabolic fates are affected by the activity of that pathway, can also act as its regulators. In living pigment cells, tyrosinase (EC 1.14.18.1), a crucial and rate-limiting enzyme of melanogenesis, acts in subcellular organelles known as melanosomes. Melanin is laid down only in these organelles. We demonstrate that supplementing Ham's F-10 medium with additional L-tyrosine or L-dopa during the culture of amelanotic Bomirski hamster melanoma cells results in a rapid increase in melanin formation, which is not simply due to greater availability of substrate. There is a rapid increase in tyrosinase activity and a large scale synthesis of melanosomes. The effects of L-tyrosine and L-dopa are prevented by the addition of cycloheximide. The actions of L-tyrosine and L-dopa are specific in that under similar conditions D-tyrosine, D-dopa, N-acetyl-L-tyrosine, L-phenylalanine, L-tryptophan and L-valine have little or no effect. The two substrates, L-tyrosine and L-dopa, appear to act through related but distinct mechanisms. Our findings provide an example of a little-known phenomenon: regulation of a differentiated eukaryotic phenotype through positive control by substrates in the pathway.
This article has been cited by other articles:
|
|
 |
|
  A. Slominski, D. J. Tobin, S. Shibahara, and J. Wortsman Melanin Pigmentation in Mammalian Skin and Its Hormonal Regulation Physiol Rev, October 1, 2004; 84(4): 1155 - 1228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Slominski, A. Pisarchik, D. J. Tobin, J. E. Mazurkiewicz, and J. Wortsman Differential Expression of a Cutaneous Corticotropin-Releasing Hormone System Endocrinology, February 1, 2004; 145(2): 941 - 950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. B. Anderson, Q. R. Rogers, and J. G. Morris Cats Require More Dietary Phenylalanine or Tyrosine for Melanin Deposition in Hair than for Maximal Growth J. Nutr., July 1, 2002; 132(7): 2037 - 2042. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tsatmali, J. Ancans, and A. J. Thody Melanocyte Function and Its Control by Melanocortin Peptides J. Histochem. Cytochem., February 1, 2002; 50(2): 125 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Sprong, S. Degroote, T. Claessens, J. van Drunen, V. Oorschot, B. H.C. Westerink, Y. Hirabayashi, J. Klumperman, P. van der Sluijs, and G. van Meer Glycosphingolipids are required for sorting melanosomal proteins in the Golgi complex J. Cell Biol., October 29, 2001; 155(3): 369 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Slominski, G. Ermak, J. E. Mazurkiewicz, J. Baker, and J. Wortsman Characterization of Corticotropin-Releasing Hormone (CRH) in Human Skin J. Clin. Endocrinol. Metab., March 1, 1998; 83(3): 1020 - 1024. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Slominski, J. Baker, T. G. Rosano, L. W. Guisti, G. Ermak, M. Grande, and S. J. Gaudet Metabolism of Serotonin to N-Acetylserotonin, Melatonin, and 5-Methoxytryptamine in Hamster Skin Culture J. Biol. Chem., May 24, 1996; 271(21): 12281 - 12286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Winder, A Wittbjer, E Rosengren, and H Rorsman The mouse brown (b) locus protein has dopachrome tautomerase activity and is located in lysosomes in transfected fibroblasts J. Cell Sci., January 9, 1993; 106(1): 153 - 166. [Abstract] [PDF]
|
|
