spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bowser, S. S.
Right arrow Articles by Rieder, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bowser, S. S.
Right arrow Articles by Rieder, C. L.

Journal of Cell Science, Vol 89, Issue 3 297-307, Copyright © 1988 by Company of Biologists

JOURNAL ARTICLES

Microtubules associate with actin-containing filaments at discrete sites along the ventral surface of Allogromia reticulopods

SS Bowser, JL Travis and CL Rieder
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.

We have investigated the distribution of actin and microtubules in pseudopodial networks (reticulopods) of the protozoan Allogromia sp., strain NF, in order to help elucidate the respective roles these components play in network organization and motility. Double-label fluorescence studies with tubulin antibodies and tetramethyl-rhodamine (TMR)-phalloidin reveal that microtubules and filamentous actin co-localize in regions where trunk pseudopods contact the substratum and splay to form the pseudopodial network; distal to these regions the network contains numerous microtubules but little or no F-actin. Similar results were obtained using various commercial actin antibodies. Correlative anti-actin immunofluorescence and high-voltage electron microscopy of serial 0.25 micron sections reveal that actin is contained within discrete electron-opaque, fan-shaped structures distributed along the cytoplasmic aspect of the ventral reticulopodial membrane. Electron microscopy of serial 100 nm sections from conventionally fixed specimens confirms that these actin-rich plaques are composed of a felt of roughly parallel, 5 nm diameter filaments. A subset of parallel and often bundled microtubules is enmeshed within, or contacts the periphery of, these filament plaques. Upon leaving a plaque, bundled microtubules frequently splay into smaller bundles. These observations are consistent with the hypothesis that interactions between microtubules and actin-containing microfilaments, particularly at substratum adhesion points, are involved in various aspects of reticulopodial motility, particularly network morphogenesis and cell body locomotion.


This article has been cited by other articles:


Home page
 Journal of Foraminiferal ResearchHome page
J. L. Travis, E. A. Welnhofer, and D. D. Orokos
AUTONOMOUS REORGANIZATION OF FORAMINIFERAN RETICULOPODIA
Journal of Foraminiferal Research, October 1, 2002; 32(4): 425 - 433.
[Abstract] [Full Text] [PDF]

Home page
 Journal of Foraminiferal ResearchHome page
S. S. Bowser and J. L. Travis
RETICULOPODIA: STRUCTURAL AND BEHAVIORAL BASIS FOR THE SUPRAGENERIC PLACEMENT OF GRANULORETICULOSAN PROTISTS
Journal of Foraminiferal Research, October 1, 2002; 32(4): 440 - 447.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1988