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Journal of Cell Science, Vol 89, 541-550, Copyright © 1988 by Company of Biologists

Submitted on July 14, 1987
Accepted on December 14, 1987

Cell biology of the stigma of Brassica campestris in relation to CO2 effects on self-pollination

PATRICIA O'NEILL 1, MOHAN B. SINGH 1, and R. B. KNOX 1

1 Plant Cell Biology Research Centre, School of Botany, University of Melbourne Parkville, Victoria 3052, Australia

The effect of carbon dioxide in blocking the sporophytic self-incompatibility system in Brassica campestris occurs within the first 2h of pollination at the pollen-stigma interface. Percentage germination of self pollen on the stigma was found to be similar in air and in the presence of 5% CO2. The CO2 effect therefore must occur after pollen germination, modifying the interaction between pollen tubes and stigma cells. Lectin binding studies showed the presence of fucosyl but not galactosyl residues on the stigma surface. Gel electrophoresis of plant extracts showed that stigma esterase activity is marked in comparison to other plant tissue. This activity is shown histochemically to be localized on the stigma cell surface and in the nucleus. Carbonic anhydrase has been detected on the stigma surface by two different histochemical methods and its possible relationship to the CO2 effect is discussed.

Key words: self-incompatibility, esterase, lectins, carbonic anhydrase

Submitted on July 14, 1987
Accepted on December 14, 1987






© The Company of Biologists Ltd 1988