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Journal of Cell Science, Vol 90, 351-364, Copyright © 1988 by Company of Biologists
Submitted on January 5, 1988
Accepted on February 18, 1988
1 Centre de Biologie Cellulaire, CNRS, 94205, Ivry-sur-Seine, France
2 Centre de Génétique Moléculaire, CNRS, 91190 Gif-sur-Yvette, France
3 Groupe de Zoologie et Prostistologie, Université de Clermont II, 63170 Aubière, France
4 Centre de Généliqne Moléculaire, CNRS, 91190 Gif-sur-Yvette, France
Author for correspondence
Among the various cytoskeletal networks and structures that compose the highly organized cortex of Paramecium, the infraciliary lattice (ICL) is the inner most one and marks the cortical boundary. It is made up of filamentous bundles running around the proximal ends of basalbodies. Immunofluorescence studies, using two different antibodies specifically labelling the network, allowed us to describe the disorganization-reorganization cycle of the ICL during division, and observations by immuno electron microscopy provided new information on it sultra structure. A partial purification of large fragments of a contracted form of the network was obtained. Among the proteins from ICL-enriched fractions, two 23 to 24(x103) Mr polypeptides were shown to cross-react with a polyclonal antibody raised against 22 to 23(x103)Mr Ca2+-binding proteins from the microfibrillar ectoplasmic-endoplasmic boundary of Isotricha and to beselectively solubilized in the presence of EGTA. The possibility that the ICL may be a new example of a non-actin contractile system foundin lower eukaryotes is discussed.
Key words: cytoskeleton, Paramecium, immunocytochemistry, contractility
Submitted on January 5, 1988
Accepted on February 18, 1988
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