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Journal of Cell Science, Vol 90, Issue 3 485-491, Copyright © 1988 by Company of Biologists

JOURNAL ARTICLES

Endoplasmin is a reticuloplasmin

GL Koch, DR Macer and FB Wooding
Medical Research Council Laboratory of Molecular Biology, Cambridge, England.

The location of endoplasmin in the endoplasmic reticulum was investigated by biochemical and immunoelectron microscopic analyses. The protein could be obtained in a soluble form by procedures that do not involve the use of any detergents. The soluble protein has the amino- and carboxy-terminal sequences of the intact molecule, showing that it has not been proteolysed. Application of the Triton X-114 phase-separation test does not reveal significant hydrophobicity in the molecule. Immunogold labelling studies on cells with a dilated endoplasmic reticulum (ER) lumen show that endoplasmin is uniformly distributed throughout the lumen, with no evidence of a preferential association with the membrane. These studies clearly demonstrate that endoplasmin is a luminal protein of the ER, i.e. a reticuloplasmin, and not an integral membrane protein.


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© The Company of Biologists Ltd 1988