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Journal of Cell Science, Vol 90, Issue 4 531-541, Copyright © 1988 by Company of Biologists

JOURNAL ARTICLES

Fracture-flip: new high-resolution images of cell surfaces after carbon stabilization of freeze-fractured membranes

C Andersson Forsman and P Pinto da Silva
Membrane Biology Section, National Cancer Institute-Frederick Cancer Research Facility, MD 21701.

We introduce fracture-flip, a method of obtaining high-resolution views of membrane surfaces. The method, a corollary of label-fracture, is based on the stabilization of the exoplasmic halves of membranes by carbon evaporation. Inversion of these casts followed by Pt/C imaging leads to new views of the cell surface at macromolecular resolution.


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© The Company of Biologists Ltd 1988