spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by BROWNLEE, C.
Right arrow Articles by PULSFORD, A. L.
Right arrow Search for Related Content
PubMed
Right arrow Articles by BROWNLEE, C.
Right arrow Articles by PULSFORD, A. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 91, 249-256, Copyright © 1988 by Company of Biologists

Submitted on June 13, 1988
Accepted on July 15, 1988

Visualization of the cytoplasmic Ca2+ gradient in Fucus serratus rhizoids: correlation with cell ultrastructure and polarity

COLIN BROWNLEE 1 and ANN L. PULSFORD 1

1 Marine Biological Association of the United Kingdom, The Laboratory Citadel Hill, Plymouth PL1 2PB, UK

Fura-2 has been used with digital image analysis to visualize and quantify the cytoplasmic Ca2+ gradient in rhizoid cells of Fucus serratus. Ca2+ was higher at the growing rhizoid tip in about 50-60% of cells studied to date. Considerable variation in the pattern of the Ca2+ gradient has been found. Verapamil reduced but did not abolish the Ca2+ gradient. Nifedipine had no effect on Ca2+ distribution during the experimental period. Removing Ca2+ from the growth medium permeabilized the plasmalemma and allowed fura-2 to efflux from the cytoplasmic compartment, permitting an assessment of the degree of sequestration of the dye into vacuoles and vesicles. Comparisons of ratio images with electron micrographs suggested that lower Ca2+ levels were associated with the nuclear region, but there was no direct correlation between Ca2+ levels and vesicle distribution in healthy cells. The role of localized differences in Ca2+ distribution in the control of polarity is discussed.

Key words: cytoplasmic Ca2+, fura-2, digital image analysis, polarity

Submitted on June 13, 1988
Accepted on July 15, 1988


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
L. Li, N. Saga, and K. Mikami
Ca2+ influx and phosphoinositide signalling are essential for the establishment and maintenance of cell polarity in monospores from the red alga Porphyra yezoensis
J. Exp. Bot., August 1, 2009; 60(12): 3477 - 3489.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. M. Coelho, A. R. Taylor, K. P. Ryan, I. Sousa-Pinto, M. T. Brown, and C. Brownlee
Spatiotemporal Patterning of Reactive Oxygen Production and Ca2+ Wave Propagation in Fucus Rhizoid Cells
PLANT CELL, October 1, 2002; 14(10): 2369 - 2381.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. Sanders, C. Brownlee, and J. F. Harper
Communicating with Calcium
PLANT CELL, April 1, 1999; 11(4): 691 - 706.
[Full Text]

Home page
 J. Cell Sci.Home page
R Pu and K. Robinson
Cytoplasmic calcium gradients and calmodulin in the early development of the fucoid alga Pelvetia compressa
J. Cell Sci., January 11, 1998; 111(21): 3197 - 3207.
[Abstract] [PDF]


© The Company of Biologists Ltd 1988